Demonstration Data |
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A MaxQuant result file of an experiment with one experimental condition and 5 time points. |
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A MaxQuant result file of an experiment with two experimental conditions and 5 time points. |
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Pre-loaded Kinase/Phosphatase Table for 25 Species |
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Pre-processing Raw Data |
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Identify the Columns needed for Data Processing |
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Identify the Intensity Columns |
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Validate Raw Data |
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Initial processing and quality control of MaxQuant data including the removal of contaminated and reverse peptides, unnecessary columns, and invalid peptides based on filtering criteria. |
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Pre-Processing for Network Analysis |
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Print a summary kinase/phosphatase table |
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Validate existing or user-uploaded Kinase/Phosphatase data |
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The regulation check based on the input parameter for the Network Analysis |
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Network Analysis |
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Network Analysis |
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Extract/Convert Processed Data |
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Extract a summary table from processed NetPhorce data |
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Extract the network inference results from processed NetPhorce data |
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Load Shiny Plot Object to R |
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Preprocessing Data for Plotting |
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Identify and Validate Provided Clusters for plotUniqueIDsHeatmaps |
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Identify provided peptide IDs for plotting |
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Confirm or List Unique IDs from the netPhorceData |
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Plotting |
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Plot the Distribution of the Intensity Data |
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Plot the Distribution of the Intensity Data |
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PCA Plot |
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Plot Heatmap Based on the Clusters Verified from the |
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Plot Heatmap Based on the Unique IDs Verified from the |
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Dotplot displaying the log2 intensity of a single phosphopeptide across the different time points and for each condition. |
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Intensity pattern of a single or multiple phosphopeptide across the time course and for each condition. |
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A VisNetwork plot of the inferred network |
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The regulation plot allows for the users to visually evaluate the chosen thresholds. |