Demonstration Data

oneConditionExample

A MaxQuant result file of an experiment with one experimental condition and 5 time points.

twoConditionsExample

A MaxQuant result file of an experiment with two experimental conditions and 5 time points.

kinasesPhosphatases

Pre-loaded Kinase/Phosphatase Table for 25 Species

Pre-processing Raw Data

confirmColumnNames()

Identify the Columns needed for Data Processing

confirmIntensityColumns()

Identify the Intensity Columns

Validate Raw Data

processData()

Initial processing and quality control of MaxQuant data including the removal of contaminated and reverse peptides, unnecessary columns, and invalid peptides based on filtering criteria.

Pre-Processing for Network Analysis

checkPreloadKinaseTable()

Print a summary kinase/phosphatase table

validateKinaseTable()

Validate existing or user-uploaded Kinase/Phosphatase data

regulationCheck()

The regulation check based on the input parameter for the Network Analysis

Network Analysis

networkAnalysis()

Network Analysis

Extract/Convert Processed Data

extractSummaryTable()

Extract a summary table from processed NetPhorce data

extractNetworkResults()

Extract the network inference results from processed NetPhorce data

convertShinyToR()

Load Shiny Plot Object to R

Preprocessing Data for Plotting

findClusters()

Identify and Validate Provided Clusters for plotUniqueIDsHeatmaps

findPeptideIDs()

Identify provided peptide IDs for plotting

findUniqueIDs()

Confirm or List Unique IDs from the netPhorceData

Plotting

plotDistribution()

Plot the Distribution of the Intensity Data

plotHistBox()

Plot the Distribution of the Intensity Data

plotPCA()

PCA Plot

plotClustersHeatmap()

Plot Heatmap Based on the Clusters Verified from the findClusters function

plotUniqueIDsHeatmaps()

Plot Heatmap Based on the Unique IDs Verified from the findUniqueIDs function

plotSinglePeptide()

Dotplot displaying the log2 intensity of a single phosphopeptide across the different time points and for each condition.

plotMultiPeptides()

Intensity pattern of a single or multiple phosphopeptide across the time course and for each condition.

plotNetPhorce()

A VisNetwork plot of the inferred network

plotRegulation()

The regulation plot allows for the users to visually evaluate the chosen thresholds.